PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF FLOWER AND STALK OF MANGIFERA INDICA (OPIORO MANGO)

PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF FLOWER AND STALK OF MANGIFERA INDICA (OPIORO MANGO)

TABLE OF CONTENT
CONTENT                                                                            
Title page                                                                              
Declaration                                                                                              
Certification                                                                                     
Dedication                                                                                                
Acknowledgment                                                                                    
Table of content                                                                            
Abstract                                                                                           

CHAPTER ONE
1.0    INTRODUCTION                                                                           
OBJECTIVE OF THIS RESEARCH                                   
1.1    LITERATURE REVIEW                                                       
1.2    GENERAL USES AND IMPORTANCE OF
MANGIFERA INDICA                                                         
1.3    MORPHLOGY OF MANGIFERA INDICA; THE TREE AND ITS
FLOWERS                                                                                     
CHAPTER TWO
2.0    MATERIALS AND METHODS                                           
2.1    MATERIALS                                                                         
2.2    EQUIPMENTS AND APPARATUS                                             
2.3    CHEMICAL REAGENTS                                                     
2.4    PHYTOCHEMICAL SCREENING (QUANTITATIVE       
ANALYSIS)                                                                           
2.5    DETERMINATION OF SAPONIN                                      
2.6    DETERMINATION OF ALKALOID                                              
2.7    DETERMINATION OF TANINS                                         
2.8    DETERMINATION OF MOISTURE CONTENT               
2.9    DETERMINATION OF TOTAL ASH                                  
2.10  CARDIAC GLYCOSIDE                                                      
2.11  TEST FOR ANTHRACENE GLYCOSIDES                      
2.12  TERPENOIDS                                                                      
2.13  pH AT 200C (10%)                                                               
CHAPTER THREE
TABLE 3.1 ORGANOLEPTIC  TEST  RESULT                         
TABLE 3.2 RESULT OF THE  PHYTOCHEMICAL  ANALYSIS
OF FLOWER AND STALK OF Mangifera Indica    
TABLE 3.3RESULT OF ANTI-BACTERIAL ACTIVITY OF TWO
GRAM POSITIVE BACTERIA IN TWO CRUDE
EXTRACTS OF FLOWER AND STALK OF
Mangifera indica                                                         
TABLE 3.4 RESULT OF ANTI-BACTERIAL ACTIVITY OF
TWO GRAM NEGATIVE BACTERIA IN TWO
CRUDE EXTRACTS OF FLOWER AND STALK
OF Mangifera indica                                                   
TABLE 3.5 RESULT OF ANTI-FUNGAL ACTIVITY OF
TWO FUNGI IN TWO CRUDE EXTRACTS
OF FLOWER AND STALK
OF Mangifera indica                                                   
TABLE 3.6 RESULT OF MINIMUM INHIBITORY
CONCENTRATION
(MIC) OF CRUDE EXTRACT OF FLOWER
AND STALK
OF Mangifera indica                                                   
CHAPTER FOUR
DISCUSSION                                                             
CONCLUSION                                                            
RECOMMENDATION                                                
REFERENCES                                                           
APPENDIX                                                                           

 ABSTRACT
Phytochemical and antimicrobial screening analysis of Mangifera indica indicated that the presence of the following moisture content 12.33% +0.0556%,  Alkaloid      7.35% +0.0291%, Tannins 18.22% +0.0070%, Saponic Glycoside 3.82% +0.0264%, Flanonoid  6.11% + 0.0158%, Phenolic glycoside 0.010% + 0.0012%, Terpenoids 5.71% + 0.01%, Antrachionic 10.59% + 0.0158%,  pH 3.84% + 0.0529%. Cardiac Glycoside  7.90% + 0.0234%. Extracts of Mangifera indica were investigated for its antifungal and antibacterial activities. The aqueous extract did not inhibit any of the test fungi, the ethanolic extract produced zones of inhibition against Staphylococcus aureus, S. pyogens, Escherichia coli, Bacilus subfilis, while C. albican and a. flavus were not inhibited.

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