THE ANTIBACTERIAL ACTIVITY OF AZADARICHTA INDICA (NEEM PLANT)
ABSTRACT
The antibacterial effect of Azadirachta indica (neem plant) against Pseudomonas aeruginosa, Klebsiella ozanae, Staphylococcus aureus and Escherichia coli was determined using the agar cup plate techniques. The phytochemical components of A. indica showed the presence of saponin and phlobatanin and the absence of alkaloids, tannis, phenolics, plycosides, flavonoids, and triterpenes, the result showed that the test organisms were susceptible to 500mg/ml, 50mg/ml and 5mg/ml of the plant extract. The minmum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined, the result showed that the MIC and MBC were 5mg and 50mg respectively for Pseudomonas aeruginosa, K. ozanae, Staphylococcus aureus and Escherichia coli. The result of the study suggest that extracts of A. indica could be suitable for the treatment of various infections caused by P. aeruginosa, K. ozanae, S. aureus and E. coli.
TABLE OF CONTENT
Title page i
Certification ii
Dedication iii
Acknowledgement iv
Abstract v
Table of Content vi-vii
CHAPTER ONE
- Introduction 1-2
- Literature review 3-7
CHAPTER TWO
- Materials and methods 8
- Collection and preparation of samples 8
- Collection of specimen 8
- Extraction of materials 8
- Phytochemical screening of extracts of A. indica 8
- Anti-microbial susceptibility test 9
- Determination of minimum inhibitory concentration (MIC) 9
CHAPTER THREE
- Result 10
- Phytochemical activities of the extracts 10
- Antimicrobial activities of the extracts 10-12
CHAPTER FOUR
4.0 Discussion 13-14
CHAPTER FIVE
5.0 Conclusion 15
References 16
